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PathHunter® U2OS FGR Activity Assay

PathHunter® CTK Activity cell lines are engineered to express the catalytic domain from CTK of interest fused to the cytokine receptor binding domain (CRBD) of a JAK kinase. The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink™ (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the receptor-PK. Activation of the cytokine receptor-PK induces receptor phosphorylation by the CTK catalytic domain. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0884C3 2 vials $10295.00

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Related Family Members:

Receptor Family
Cytosolic Tyrosine Kinase
Coupling
N/A
Accession Number
NM_000163.2
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Recombinant Human Growth Hormone
Antagonist/Inhibitor
SKI-606
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then pre-incubated with inhibitor, followed by stimulation with the control agonist . Following agonist stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2594.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $339.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $288.00
93-0563R16A AssayComplete™ Cell Plating 16 Reagent 100 mL $288.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $236.00
92-0029 AssayComplete™ Hygromycin B 20 mL $175.00
92-0030 AssayComplete™ G418 20 mL $133.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $92.00
92-0028 AssayComplete™ Puromycin 1 mL $72.00