PathHunter® U2OS FLT3 Activity Assay

PathHunter® RTK Activity cell lines are engineered to express a fusion of the intracellular catalytic domain from the target RTK and the extracellular ligand binding domain of EGFR. This fusion protein is tagged with ProLink™ (PK). The cells co-express an Enzyme Acceptor (EA) tagged SH2 domain. Ligand induced cross-phosphorylation of the PK-tagged fusion protein leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal.

Catalog No. Size Price Quantity
93-0901C3 2 vials $10299.00

For account specific pricing, please contact Customer Service at customerservicedrx@eurofins.com.

Related Family Members:

Receptor Family
Receptor Tyrosine Kinase
Coupling
N/A
Accession Number
NM_005228.3
Species
Human
Prolink™ Tag
PK1
Cell Background
U2OS
Agonist/Ligand
Recombinant Human EGF
Antagonist/Inhibitor
PKC-412
Cells were plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells were then pre-incubated with inhibitor. Following agonist stimulation, signal was detected using the PathHunter® detection kit according to the recommended protocol.
  • [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay

    PathHunter Receptor Tyrosine Kinase (RTK) Functional Assays are functional cell-based assays used for identification, screening or profiling of agonists, small molecule inhibitors and therapeutic biologics (peptides, proteins and various types of antibodies). Additionally, they have non-R&D applications as bioassays for QC testing and testing for anti-drug ...

    [User Manual] PathHunter® Receptor Tyrosine Kinase Functional Assay
  • [User Manual] PathHunter® Receptor Tyrosine Kinase Activity Assay

    PathHunter Receptor Tyrosine Kinase (RTK) Activity Assays are functional cell-based assays used for identification, screening, or profiling of small molecule inhibitors. This assay format cannot be used for functional testing of biologics.

    [User Manual] PathHunter® Receptor Tyrosine Kinase Activity Assay

Please refer to the datasheet above for a complete list of required and recommended products.

Select Required Products

Cat. No. Description Size Price Quantity
93-0001 PathHunter® Detection Kit 75 mL (10 plates), 1,000/4,000 dp (96-well/384-well) $2619.00
92-3103G AssayComplete™ Cell Culture Kit-103 Kit $349.00
92-5103FR AssayComplete™ Freezing Reagent F3 2 x 50 mL $299.00
93-0563R16A AssayComplete™ Cell Plating 16 Reagent 100 mL $299.00
92-4103TR AssayComplete™ Thawing Reagent T3 100 mL $239.00
92-0029 AssayComplete™ Hygromycin B 20 mL $179.00
92-0030 AssayComplete™ G418 20 mL $139.00
92-0009 AssayComplete™ Cell Detachment Reagent 50 mL $99.00