Each DiscoverX cell line has been optimized for use with a specific AssayComplete Cell Plating Reagent to produce the highest possible assay performance. Each cell plating reagent was developed to maintain high cell viability and maximize ligand responsiveness for the duration of the cell-based assay while minimizing high basal activity by eliminating additional hormones and growth components that could impact the assay. These reagents are only to be used when preparing assay plates and are not intended for use during routine growth and passaging of DiscoverX cell lines.