The KILR Detection Kit is to be used with the KILR Assays for Adherent or Suspension Cell Lines when running ADCC or other immune assays that result in cell lysis. The kit is easy-to-use and has been successfully run in 96-well microplate format. The resulting signal is chemiluminescent and is read with any standard plate reader. KILR Assay products utilize the Enzyme Fragment Complementation (EFC) technology for studying cell cytotoxicity with the aid of the complementation of two β-galactosidase (β-gal) fragments. The larger β-gal fragment is called Enzyme Acceptor (EA) and the smaller fragment is termed Enzyme Donor (ED; also known as enhanced ProLabel® [ePL]). The two fragments are inactive when apart. However, when they complement, they form a functional enzyme that hydrolyzes the substrate to generate a chemiluminescent signal. The detection reagent contains the EA fragment of β-gal, and therefore must be used in conjunction with KILR cell lines, which express the ePL-tagged KILR Reporter Protein in order to obtain a detectable signal.