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5-hydroxytryptamine receptor 1B (5HT1B) - HTR1B

Target Class GPCR Family5-Hydroxytryptamine
SpeciesHuman   
Background
The neurotransmitter serotonin (5-hydroxytryptamine; 5-HT) exerts a wide variety of physiologic functions through a multiplicity of receptors and may be involved in human neuropsychiatric disorders such as anxiety, depression, or migraine. These receptors consist of several main groups subdivided into several distinct subtypes on the basis of their pharmacologic characteristics, coupling to intracellular second messengers, and distribution within the nervous system (Zifa and Fillion, 1992 [PubMed 1359584]). The serotonergic receptors belong to the multigene family of receptors coupled to guanine nucleotide-binding proteins.[supplied by OMIM, Oct 2009]

Cell Background
CHO-K1
Readout
cAMP
Method
Detect changes in intracellular cAMP levels in response to agonist stimulation of the receptor
Agonist/Ligand
5-HT
Agonist Effect (Activation)
cerebral and coronary artery vasoconstriction; increase BP
Antagonist Effect (Inhibition)
increase aggression
Ref.
Bowes et. al. 2012
Cells are plated in a 384-well plate and incubated overnight at 37°C/5% CO2 to allow the cells to attach and grow. Cells are then stimulated with a control agonist + forskolin. Following stimulation, signal is detected using the HitHunter® cAMP detection kit according to the recommended protocol.
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