[User Manual] cAMP Hunter Semaglutide Bioassay Kit

[User Manual] cAMP Hunter Semaglutide Bioassay Kit
Version:
70-441

File Name/Number:
70-441

Year:
2022

The cAMP Hunter Semaglutide Bioassay Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay asseses ligand (e.g. Exendin-4) based on activation of GLP1R GPCR activity via detection of cAMP. Activation of GLP1R either stimulates or inhibits adenylate cyclase to modulate cellular cAMP levels and produces EFC signal.This assay has been optimized and qualified with Ozempic® (not supplied in the bioassay kit). Ozempic® is a registered trademark of Novo Nordisk A/S. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.