Functional Cell-based Assays for Characterization and Development of Agonistic Antibodies as Therapeutics for Cancer and Autoimmune Diseases

Agonistic antibodies are specialized antibodies that bind and engage with their intended target to activate the target and signal a biological process. This application note describes cell-based assays to drive accurate characterization and clinical development of agonistic antibody therapeutics targeting checkpoint receptors. The indispensable role of FcR-mediated antibody clustering in capturing the agonist potential of antibodies is demonstrated.