Biological Information | |
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Background Information: | The cAMP Hunter™ GPR109B eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 3-hydroxyoctanoic acid) based activation of GPR109B GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR109B, a naturally Gi/Go coupled GPCR. Upon activation of GPR109B, the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. |
Target Class: | GPCR |
Family: | Hydroxycarboxylic Acid Receptors |
Sub Family: | Class A |
Coupling: | Gi/Go |
Accession Number: | NM_006018 |
Target Name: | GPR109B |
Target Aliases: | Hydroxycarboxylic acid receptor 3 |
Target Species: | Human |
Cell Background: | CHO-K1 |
Usage | |
Product Type: | eXpress Assay Kits |
Application: | Drug Discovery & Development |
Storage Conditions: | Store in vapor phase of liquid nitrogen. |
Usage Disclaimer: | Ready-to-use eXpress assay kits are intended for in vitro research purposes only. These kits are not suitable for lot release applications. Refer to the ready-to-use bioassay kit format for potency testing in lot release for biologic therapeutics. These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Type: | Functional |
Assay Measures: | cAMP Detection |
Detection Method: | Chemiluminescence |
Additional Information | |
Brand: | cAMP Hunter™ |
cAMP Hunter™ eXpress GPR109B CHO-K1 GPCR Assay
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The cAMP Hunter™ eXpress GPR109B CHO-K1 GPCR Assay measures GPR109B (GPCR) activity via decreased levels of intracellular cAMP. eXpress kits contain all assay materials: cells, reagents, and plates.
User Manuals & Protocols
70-234 cAMP Hunter eXpress GPCR Assay REV10 User Manual
View DocumentDatasheets
95-0141E2x Datasheet
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