| Biological Information | |
|---|---|
| Background Information: | The ChemiScreen™ Chem-2 Parental Control Membrane Preparation is a membrane prep derived from ChemiScreen parental cells. This Chem-2 based cell line lacks most endogenous GPCR expression, and expresses high levels of the promiscuous G protein Gα15, allowing forced coupling of any class of GPCR to calcium signaling pathways. This membrane prep is intended for use as a negative control alongside ChemiScreen GPCR membrane preps of interest in radioligand binding assays or GTPγS assays. Membrane preps are crude preparations of membranes derived from a stably modified clonal cell line which was chosen for optimal receptor expression. |
| Target Class: | GPCR |
| Cell Background: | CHO-K1 |
| Usage | |
| Product Type: | Membrane Preparations |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Vial to be stored at -70°C. Avoid repeat freeze/thaw cycles. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | FluorescenceRadioactivity |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
ChemiScreen™ Chem-2 Parental Control Membrane Preparation
The ChemiScreen™ Chem-2 Parental Control Membrane Preparation is a membrane prep derived from Chem-2 ChemiScreen parental cells, expressing high levels of Gα15. Membrane preps contain crude membranes from a stably modified cell line.
Datasheets
HTS000MC2 Datasheet
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