| Biological Information | |
|---|---|
| Background Information: | The Ready-to-Assay™ IP1 Prostanoid Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. prostaglandin I2) based activation of PTGIR GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PTGIR alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PTGIR , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
| Target Class: | GPCR |
| Family: | Prostanoid Receptors |
| Accession Number: | AY242134 |
| Target Name: | PTGIR |
| Target Aliases: | Prostaglandin I2 (prostacyclin) receptor (IP), PTGIR, IP, Prostanoid |
| Target Species: | Human |
| Usage | |
| Product Type: | Frozen Cells |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
Ready-to-Assay™ IP1 Prostanoid Receptor Frozen Cells
The Ready-to-Assay™ IP1 Prostanoid Receptor Frozen Cells measure PTGIR (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
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