Biological Information

Background Information:

The Ready-to-Assay P2Y1 Purinergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. ADP, ATP) based activation of P2RY1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress P2RY1 , a naturally Gq coupled GPCR. Upon activation of P2RY1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

P2Y

Sub Family:

Class A

Protein Name:

P2Y1

Protein Aliases:

ADP receptor|Purinergic receptor

Accession Number:

NM_002563.2

UniProt Number:

P47900

Gene Name:

P2RY1

Gene ID:

5028

Gene Aliases:

P2Y1

Target Species:

Human

Cell Background:

1321N1

Usage

Product Type:

Frozen Cells

Application:

Drug Discovery & Development

Storage Conditions:

Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.

Usage disclaimer:

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Assay Information

Assay Type:

Cell Based

Assay Measures:

Calcium Flux

Detection Method:

Fluorescence

Additional Information

Brand:

ChemiSCREEN™