| Biological Information | |
|---|---|
| Background Information: | The Ready-to-Assay™ P2Y1 Purinergic Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. ADP, ATP) based activation of P2RY1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress P2RY1 , a naturally Gq coupled GPCR. Upon activation of P2RY1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
| Target Class: | GPCR |
| Family: | P2Y Receptors |
| Accession Number: | NM_002563.2 |
| Target Name: | P2RY1 |
| Target Aliases: | Purinergic receptor P2Y, G-protein coupled, 1 |
| Target Species: | Human |
| Usage | |
| Product Type: | Frozen Cells |
| Application: | Drug Discovery & Development |
| Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
| Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
| Assay Information | |
| Assay Measures: | Calcium Flux |
| Detection Method: | Fluorescence |
| Additional Information | |
| Brand: | ChemiSCREEN™ |
Ready-to-Assay™ P2Y1 Purinergic Receptor Frozen Cells
The Ready-to-Assay™ P2Y1 Purinergic Receptor Frozen Cells measure P2RY1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
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