Biological Information | |
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Background Information: | The Ready-to-Assay™ PAC1-long Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. PACAP) based activation of ADCYAP1R1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress ADCYAP1R1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of ADCYAP1R1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
Target Class: | GPCR |
Family: | VIP and PACAP Receptors |
Accession Number: | AY366498, NM_001118 |
Target Name: | ADCYAP1R1 (PAC1 receptor) |
Target Aliases: | Adenylate cyclase activating polypeptide receptor 1, ADCYAP1R1, PAC1, VIP & PACAP |
Target Species: | Human |
Usage | |
Product Type: | Frozen Cells |
Application: | Drug Discovery & Development |
Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
Ready-to-Assay™ PAC1-long Receptor Frozen Cells
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The Ready-to-Assay™ PAC1-long Receptor Frozen Cells measure ADCYAP1R1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
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