Biological Information
Background Information:
The Ready-to-Assay PK1 Prokineticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Prokineticin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PROKR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.
Target Class:
GPCR
Family:
Prokineticin
Sub Family:
Class A
Protein Name:
PK1
Protein Aliases:
G-protein coupled receptor 73|G-protein coupled receptor ZAQ
Accession Number:
NM_138964
UniProt Number:
Q8TCW9
Gene Name:
PROKR1
Gene ID:
10887
Gene Aliases:
PKR1|ZAQ|GPR73a|GPR73
Target Species:
Human
Cell Background:
Chem-1
Usage
Product Type:
Frozen Cells
Application:
Drug Discovery & Development
Storage Conditions:
Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C.
Usage disclaimer:
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Assay Information
Assay Type:
Cell Based
Assay Measures:
Calcium Flux
Detection Method:
Fluorescence
Additional Information
Brand:
ChemiSCREEN™