Biological Information | |
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Background Information: | The Ready-to-Assay™ PK1 Prokinecticin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Prokinecticin, EG-VEGF) based activation of PROKR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress PROKR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of PROKR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format. |
Target Class: | GPCR |
Family: | Prokineticin Receptors |
Accession Number: | NM_138964 |
Target Name: | PROKR1 |
Target Aliases: | Prokineticin receptor 1, GPR73, GPR73a, PK-R1, PKR1, ZAQ |
Target Species: | Human |
Usage | |
Product Type: | Frozen Cells |
Application: | Drug Discovery & Development |
Storage Conditions: | Short term (24 h) in vapor phase of liquid nitrogen. Media at 4°C. |
Usage Disclaimer: | These products may be covered by issued US and/or foreign patents, patent application and subject to Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information. |
Assay Information | |
Assay Measures: | Calcium Flux |
Detection Method: | Fluorescence |
Additional Information | |
Brand: | ChemiSCREEN™ |
Ready-to-Assay™ PK1 Prokinecticin Receptor Frozen Cells
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The Ready-to-Assay™ PK1 Prokinecticin Receptor Frozen Cells measure PROKR1 (GPCR) activation via an increase in intracellular calcium. Ready-to-Assay kits contain two vials of thaw-and-use cells.
User Manuals & Protocols
70-427 ChemiSCREEN and ChemiBRITE Cell Lines User Manual REV1
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HTS074RTA Datasheet
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