Control Ligands and Inhibitors

Ligands are small or large molecules that can bind to transmembrane receptor or intracellular protein targets such as enzymes and alter the state of the target molecule. These ligands can be a variety of molecules including small molecule chemicals, proteins such as growth factors and cytokines, as well as antibodies. In drug discovery and development, ligands are often used as probes to determine the ligand's mechanism-of-action (MOA), understand the target's function, or identify and screen potential drug targets.

 

Ligands can be classified as agonists, partial agonists, super agonists, allosteric modulators, or antagonists, depending on whether they activate, change, or inhibit the target protein they bind. When activating ligands bind to a target, the structure is altered. The binding may lead to the dimerization of a receptor and/or initiation of a cascade of cellular signal transduction events ultimately leading to a unique cellular response or phenotype. Many such cellular responses are vital for the proliferation, migration, survival, and differentiation of cells within all multicellular organisms. In contrast, inhibitor ligands typically block signaling. Inhibitors of intracellular proteins like enzymes fall under the reversible or irreversible types. While both types bind to the target protein, the reversible inhibitors dissociate from the target under certain conditions, whereas irreversible inhibitors do not. Overall, inhibitors play a significant role in drug discovery and are used to analyze cellular pathways and treat various disorders.

 

Choose from a variety of small molecules, peptides, and recombinant proteins as control ligands and inhibitors as well as antibodies and antibiotics that support Eurofins DiscoverX cell-based assays for developing your target-based therapeutics development.

 

 

 

Advantages of Ligands and Inhibitors

  • Validated – Pre-validated reference ligands and inhibitors conveniently supplied with a recommended reconstitution buffer for use with Eurofins DiscoverX cell-based assays
  • Extensive Menu – Access over 300 control ligands and inhibitors, including small molecules, peptides, and recombinant proteins, to support small molecule or biologic drug discovery and development
  • Diverse Applicability – Determine ligands MOA, understand the function of targets, or identify and screen potential drug targets

Related Topics

  • Cells & Cell Lines – Stable cell lines, cell pools, frozen cells, and primary effector cells for a broad range of target classes, including GPCRs, kinases, checkpoint receptors, ion channels, cytokines, epigenetics, nuclear proteins, and many other target classes
  • Detection Kits and Reagents – Eurofins DiscoverX detection products are designed for sensitive quantification of biologics or small molecules to provide accurate pharmacology and reproducible results
  • Custom Capabilities – Custom cell lines, kits, assays, and protein development capabilities optimized to fit your requirements
  • Toolbox Products – Complete set of parental cell lines, expression and cloning vectors, kits, and retroparticles to build your own stable cell lines and cell-based assays
  • Recombinant Enzymes and Proteins – Over 500 high-quality and purified recombinant kinases, phosphatases, and epigenetic and ubiquitin proteins produced in their optimal system (insect, mammalian, or bacterial) to give the correct characteristics for activity and inhibition

Products

  • Ligands and Inhibitors – More than 330 control ligands and inhibitors, including small molecules, peptides, and recombinant proteins to support your Eurofins DiscoverX cell-based assays.
  • PathHunter Antibodies – High-quality monoclonal antibodies specific for Enzyme Fragment Complementation (EFC) β-galactosidase fragments, enzyme donors ProLabel® and ProLink™ and enzyme acceptor, and optimized for a broad range of applications including Western Blot and immunofluorescence.

Access Products by Product Type and Ligand Name