Custom Assay Development Services

Assay development solutions tailored to your needs

When existing off-the-shelf solutions are not enough, trust DiscoverX to develop a solution that will produce the quality results you expect. DiscoverX offers a range of custom development services including bioassays for drug discovery, process development and basic research. Our team of molecular and cellular biologists will work with you to match one of our proprietary platforms such as PathHunter®, KINOMEscan®, InCell Hunter® or HitHunter® to deliver a cellular or biochemical solution that provides the quality results you expect.

Available Services

  • Orthologs and mutants
  • Custom cell backgrounds
  • Custom cell lines
  • Protein overexpression
  • Kit formulation/packaging

Contact DiscoverX Custom Assay Development Services Team to learn more


 


 
 

For more information about our services, select one of the tabs below.


PathHunter® Cell-based Assay Development Services

    PathHunter is a versatile cell-based assay platform, based on our proprietary EFC technology, successfully employed to develop validated assay solutions for over 300 targets spanning many target classes including GPCRs, Kinase, NHRs, and more. Innovative application of this core technology, has enabled the development of robust, reliable, and high-throughput-friendly chemiluminescent assays to measure protein-protein interaction, protein translocation and degradation.
PathHunter CAD Services will work closely with you to develop a custom cell-based assay specific for your target, providing comprehensive solutions where you need them most.

PathHunter - cell-based technology format can query many types of interactions

  • Measure inducible and constitutive protein interactions
  • Measure protein translocation to various intracellular locations in a
    non-imaging HTS friendly assay
  • Accurately measure protein levels and stabilization
How it Works

Engineered Cell Lines

A flexible approach to customized cell line generation
Using proprietary DiscoveRx vectors and viral transduction techniques, we can build custom engineered cell lines specific to your project requirements in as little as 6-8 weeks. Need a modified cell background for an existing catalog product, mutant or ortholog? These can be fast-tracked and in certain cases developed in as little as 3 weeks from project acceptance.

Capabilities

  • Cell background variants
  • Mutant or orthologs of existing catalog products
  • Protein overexpression
  • Bulk cell-scale up

Contact the Custom Assay Development Services Team to learn more >
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Custom PathHunter® Assay Development Solutions

Comprehensive flexible cell-based assay solutions
When you need more than just a cell line, you can count on our custom assay development team to develop and validate a comprehensive assay solution. Our team of research scientists will work closely with you to determine project scope, feasibility, time-lines and budget to develop a workplan that meets your needs.

Flexible Partnership and Development Options

  • Low up-front cost
  • Routine updates
  • Flexible business models: fee-for-service, milestone driven and more

Contact DiscoveRx Custom Assay Development Services Team to learn more >
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HitHunter® Biochemical Assay Development Services

    The HitHunter Assay is a validated platform for biochemical quantification of second messenger signaling for cAMP and cGMP. Extension of the HitHunter principle to novel targets allows for investigation of protease and substrate activity.  HitHunter assays are formatted as competitive immunoassays, where analytes are detected in solution, without separation or wash steps, providing improved sensitivity and precision compared to conventional immunoassays.

HitHunter Biochemical Assay Development Services will work closely with you to develop a custom assay to detect protease and substrate activity or measure an analyte, providing comprehensive solutions where you need them most.

HitHunter biochemical technology format has these advantage

  • Develop novel assays for identifying analytes
  • Develop cleavage assays to assay protease activity
  • Develop assays for use with primary cells, etc
  • All assays will be HTS compatible, etc.
  • Chemiluminescent detection - minimizes false positives from auto fluorescence
  • Reliable and robust assay with high Z' (0.75)
  • HTS-friendly - adaptable to 1536 applications
  • Large assay windows
  • Broad application to multiple target classes
How it Works

Custom HitHunter™ Assay Development Solutions

An award winning, homogenous assay platform for precise, sensitive analyte detection
Using proprietary DiscoveRx technology, we can generate custom protease cleavage assays that utilize ED conjugated to the protease specific cleavage sequences as a substrate proteases.

Capabilities

  • Monitoring Gi and Gs targets
  • Identifying agonist, antagonist, allosteric, partial agonists and inverse agonists
  • High-throughput screening (HTS & uHTS) campaigns
  • Ligand profiling
  • Use with primary cells, orphans, frozen and membrane
  • Identifying PDE inhibitors

Contact the Custom Assay Development Services Team to learn more >
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InCell Hunter™ Assay Development Services

    InCELL Hunter is a novel, yet powerful in vitro cellular ligand binding assay platform that can be used to determine compound binding in a native cellular environment. InCELL Hunter uses enzyme fragment complementation technology to measure binding to the protein of interest and is a powerful development tool for intractable targets where other technologies have been unsuccessful.

InCell Hunter – A simple cell-based assay for complex targets

  • Cell-based assay broadly applicable to several target classes
  • Target-specific response
  • Direct measure of intracellular binding to target
  • Activity independent
  • HTS compatible
  • Bridge between in vitro binding assay and cellular functional assay
How it Works

InCELL Hunter™ Assay Development Solutions

Intracellular Binding Assays - measure cellular potencies for your compounds
We can build custom engineered cell lines specific to your project requirements in as little as 6-8 weeks using the InCell Hunter™ technology platform.

Capabilities

  • Intractable targets
  • Cell background variants
  • Mutant or orthologs of existing catlaog products
  • Bulk cell-scale up

Contact the Custom Assay Development Services Team to learn more >
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