[User Manual] KILR® Cytotoxicity Assay−Adherent Target Cell Lines
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The KILR cytotoxicity cell line enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody-Dependent Cell-mediated Cytotoxicity, ADCC, Complement-Dependent Cytotoxicity (CDC), Antibody-Dependent Cell Phagocytosis (ADCP), Cytotoxic T Cell Lymphocyte-Mediated Death (CTL), Bi-specific Antibody Mediated T Cell Redirection, Chimeric Antigen Receptor T Cell (CAR-T), and adoptive T cell therapies. This assay can drive all aspects of the drug discovery process, from screening to characterization, and the development of a QC lot-release assay for the immunotherapy drugs.
Each KILR Assay target clonal cell line or stable pool has been characterized for its stability in culture, and has been found to be stable for at least 15 passages. Assays should be run using fresh, low-passage cells that are seeded and rapidly followed by opsonization with antigen-specific antibodies.