[User Manual] KILR® Retroparticles For Cytotoxicity Assays

[User Manual] KILR® Retroparticles For Cytotoxicity Assays
Version:
042817

File Name/Number:
70-373

Year:
2017

The KILR Retroparticles provide a potent vehicle for the stable delivery of the KILR reporter construct into almost any dividing cell line, enabling the creation of KILR cytotoxicity assays in the cell line expressing the target of your choice. The KILR Cytotoxicity cell line you generate enables the development of a simple, non-radioactive and dye-free method to specifically measure target cell death in a co-culture. The KILR assay has several applications in iOnc Cancer Immunotherapy (iOnc) drug development including measuring target cell death through Antibody Dependent Cellmediated Cytotoxicity (ADCC), Complement Dependent Cytotoxicity (CDC), Antibody Dependent Cell Phagocytosis (ADCP), cytotoxic T cell lymphocyte mediated death (CTL), Bi-specific Antibody Mediated T Cell Redirection, Chimeric Antigen Receptor T cell (CAR-T) and adoptive T cell therapies. This assay can drive all aspects of the drug discovery process, from screening to characterization, and the development of a QC lot release assay for the immunotherapy drugs.