[User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines

[User Manual] PathHunter® β-Arrestin Assay for GPCR Cell Lines
Year:
2018

PathHunter β-Arrestin cell lines are stable clonal cell lines that expedite drug discovery and development by providing robust response to over 90% of all known G-protein coupled receptor (GPCRs), with accurate pharmacology. This assay measures an essential pathway in GPCR activation, i.e. β-arrestin recruitment to activated GPCRs, enabling scientists to screen for and profile functional agonists and inhibitors of GPCRs. These assays are successfully used to identify and optimize biologics and small molecule drugs, in addition to being used to develop potency assays for the QC lot release testing of numerous biologic drugs. Since β-arrestin recruitment occurs independent of G-protein coupling, these assays provide a direct, universal platform for measuring receptor activation.