[User Manual] PathHunter® Activated GPCR Internalization Assay
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PathHunter® Activated GPCR Internalization Assays are non-imaging, non-antibody based chemiluminescent detection assays that provide a direct and quantitative measurement of internalized GPCRs localized to early endosomes. This allows the internalization of GPCRs to be quantitatively measured in live cells without the need for expensive microscopy. The PathHunter system combines engineered clonal cell lines with optimized PathHunter Detection Reagents (DiscoverX, Cat. # 93-0001 Series). Each cell line has been characterized for appropriate GPCR pharmacology, specificity and stability in cell culture. Whether you are studying recep-tor recycling, identifying functional antagonists, or determining mechanism of action of your lead compounds, a simple, one-step addition protocol and standard chemiluminescent detection makes these assays ideally suited for 96-well, 384-well, or 1536-well compound screening.