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[User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit

[User Manual] PathHunter® Jurkat SIRPα Signaling Bioassay Kit
Version:
Rev 2

File Name/Number:
70-411

Year:
2020

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The PathHunter SIRPα Signaling Bioassay Kit provides a simple, highly specific assay to enable discovery and development of small molecule and biologic drugs targeting the CD47-SIRPα signaling axis. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control antibody, and assay plates. The cryopreserved cells have been manufactured to ensure longterm assay reproducibility, and faster implementation from characterization to QC lot release. This bioassay has been optimized for a 96-well format. The protocol can be optimized further for running the assay in a highthroughput 384-well format.