Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.
Target engagement assays measure compound binding to a specific protein target. In a cellular environment, these assays monitor compound cellular penetration and compound-target interaction to enable the assessment of compound efficacy and confirmation of mode-of-action.
InCELL cell-based compound-target engagement assays provide the ability to confirm compound cell entry and protein target binding, screen and rank inhibitors, validate hits identified in biochemical assays, and measure cellular EC50 values all in the native cellular environment. These simple binding assays require no custom chemical tracers nor antibody reagents and provide a convenient solution when functional assays are difficult or unavailable.
Access two leading Eurofins DiscoverX® assay platforms, InCELL Pulse™ and InCELL Hunter™ — both based on the industry validated EFC technology with similar underlying principles, but crucial differences that suit them for different protein targets. The InCELL Pulse platform is built on the principle of protein thermal stability, while InCELL Hunter is built on the principle of protein stabilization related to protein turnover or interaction disruption.
InCELL target engagement assays are a perfect complement to Targeted Protein Degradation (TPD) programs that allow you interrogate and optimize target-binding portion. Discover the power of the InCELL assays and take advantage of stable cell lines, ready-to-use kits, and a do-it-yourself, starter kit perfect for creating your own compound-target engagement assay.
Consider Eurofins DiscoverX’s custom development capabilities for custom cell lines, assays, & enzyme development.
Eurofins DiscoverX offers two target engagement assay platforms, InCELL Pulse and InCELL Hunter. The InCELL Hunter platform offers researchers cell lines, ready-to-use kits and profiling services for specific targets. The InCELL Pulse platform offers researchers ready-to-use kits for specific targets and a do-it-yourself, starter kit perfect for creating your own compound-target engagement assay.