ChemiBRITE FSH Glycoprotein Hormone Receptor Stable Cell Line

Background Information:

The ChemiBRITE FSH Glycoprotein Hormone Receptor cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. follicle stimulating hormone) based activation of FSHR GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses FSHR alongside exogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. ChemiBRITE cells also express a clytin variant, allowing for luminescent as well as fluorescent signal detection. Upon activation of FSHR , the resulting calcium activity can be assayed in either detection mode.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Glycoprotein Hormone

Sub Family:

Class A

Protein Name:

FSH

Protein Aliases:

Follitropin receptor

Accession Number:

NM_000145

UniProt Number:

P23945

Gene Name:

FSHR

Gene ID:

2492

Gene Aliases:

FSHRO|LGR1

Target Species:

Human

Cell Background:

HEK293