ChemiSCREEN™ CRF1 Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ CRF1 Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. CRH, sauvagine) based activation of CRHR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses CRHR1 alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of CRHR1 , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Corticotropin-Releasing Factor

Sub Family:

Class B

Protein Name:

CRF1

Protein Aliases:

CRF-R1|CRFR-1|Corticotropin-releasing hormone receptor 1|CRH-R-1|CRH-R1

Accession Number:

X72304

UniProt Number:

P34998

Gene Name:

CRHR1

Gene ID:

1394

Gene Aliases:

CRF-R|CRF1

Target Species:

Human

Cell Background:

Chem-1