InCELL Hunter™ HEK 293 PI3Kδ Kinase Binding Cell Line

Background Information:

The InCELL Hunter™ HEK 293 PI3Kδ Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PI3Kδ via detection of PI3Kδ levels. This product uses EFC technology. The cell line included overexpresses PI3Kδ target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

PtdIns(4,5)P 3-Kinase

Sub Family:

Lipid

Protein Name:

PI3Kdelta

Protein Aliases:

phosphatidylinositol 3-kinase, catalytic, delta polypeptide|phosphoinositide-3-kinase C

Accession Number:

NM_005026.3

UniProt Number:

O00329

Gene Name:

PIK3CD

Gene ID:

5293

Gene Aliases:

p110D

Target Species:

Human

Cell Background:

HEK293