InCELL Hunter™ HEK 293 PIKFYVE Kinase Binding Cell Line

Background Information:

The InCELL Hunter™ HEK 293 PIKFYVE Kinase Binding Cell Line is a stable, engineered cell line for use in studying small molecule drug candidates that engage with kinases. This cell line enables assessment of compound-engagement-based stabilization of PIKFYVE via detection of PIKFYVE levels. This product uses EFC technology. The cell line included overexpresses PIKFYVE target protein fused to PL. In the absence of compound that binds and stabilizes the target, this protein is denatured by the heat treatment and no EFC signal is produced. When compound engages and heat-stabilizes the target, EA contained in the detection reagent produces EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

1-PtdIns 5-Kinase

Sub Family:

Lipid

Protein Name:

PIKFYVE

Protein Aliases:

FYVE finger-containing phosphoinositide kinase|PIKfyve|Phosphatidylinositol 3-phosphate 5-kinase type III|PIPkin-III|Type III PIP kinase

Accession Number:

NM_015040.3

UniProt Number:

Q9Y2I7

Gene Name:

PIKFYVE

Gene ID:

200576

Gene Aliases:

KIAA0981|PIP5K3

Target Species:

Human

Cell Background:

HEK293