Biological Information

Background Information:

The PathHunter® CHO-K1 GLP1 (7-37) Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses GLP1 (7-37) based activation of GLP1R GPCR via detection of β-arrestin recruitment. The included cells overexpress ProLink-tagged GLP1R and EA-tagged β-Arrestin-2. Activation of GLP1R stimulates the recruitment of β-Arrestin-2 and produces EFC signal. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Bioassay Kits

Application:

Potency, Commercial Release & Stability

Kit Components:

KitComponent

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

β-Arrestin Recruitment

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

PathHunter®