Biological Information

Background Information:

The PathHunter® GLP1R Activated GPCR internalization cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This cell line enables assessment of ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The included cell line overexpresses EA-tagged β-arrestin 2 as well as a PK-tagged early endosome protein and untagged GLP1R. Upon ligand-induced receptor activation, β-arrestin 2 is recruited to the activated GPCR and the resulting GLP1R-arrestin complex is internalized to the early endosome, leading to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

β-Arrestin Isoform:

β-Arrestin-2

Assay Measures:

Internalization

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

PathHunter®