PathHunter® Ranibizumab Bioassay HS Kit

Background Information:

The PathHunter® Ranibizumab Bioassay HS Kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. VEGF-165) based activation of KDR/KDR Kinase activity via detection of Dimerization. The included cells overexpress ProLink-tagged KDR/KDR and EA-tagged. Activation of KDR/KDR stimulates the recruitment of and produces EFC signal. This assay has been optimized and qualified with Lucentis® (not supplied in the bioassay kit). Lucentis® is a registered trademark of Genentech, Inc.. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates. The HS kit has been optimized to deliver reproducible results with lower variability.

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Target Class:

Kinase

Family:

RTK

Sub Family:

Protein Tyrosine

Protein Name:

KDR (VEGFR2)

Protein Aliases:

vascular endothelial growth factor receptor 2|fetal liver kinase 1

Accession Number:

NM_002253.2

UniProt Number:

P35968

Gene Name:

KDR

Gene ID:

3791

Gene Aliases:

FLK1|VEGFR|VEGFR2|CD309

Target Species:

Human

Cell Background:

HEK293