PathHunter® eXpress GCGR Total GPCR Internalization Assay

Background Information:

The PathHunter® GCGR Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. glucagon) based internalization of GCGR via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress EA-tagged GCGR and a PK-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GCGR receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

Glucagon

Accession Number:

NM_000160

UniProt Number:

P47871

Gene Name:

GCGR

Gene ID:

2642

Gene Aliases:

GGR

Target Species:

Human

Cell Background:

HEK293