PathHunter® eXpress GLP1R Total GPCR Internalization Assay

Background Information:

The PathHunter® GLP1R Total GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates affecting GPCR activation and internalization. This kit assesses ligand (e.g. exendin-4) based internalization of GLP1R via detection of localization to the early endosome. This product uses EFC technology. The cells included overexpress PK-tagged GLP1R and an EA-tagged early endosome protein. Upon ligand-induced receptor activation, resulting internalization of the GLP1R receptor to the early endosome leads to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Coupling:

Gs

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

U2OS