Ready-to-Assay AT1 Angiotensin Receptor Frozen Cells

Background Information:

The Ready-to-Assay AT1 Angiotensin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. Angiotensin-II) based activation of AGTR1 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress AGTR1 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of AGTR1 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Angiotensin

Sub Family:

Class A

Protein Name:

AT1

Protein Aliases:

AT1BR|Angiotensin II type-1 receptor|AT1

Accession Number:

NM_000685

UniProt Number:

P30556

Gene Name:

AGTR1

Gene ID:

185

Gene Aliases:

AT1|AT2R1|AGTR1A|AT2R1A|HAT1R|AG2S|AT2R1B|AT1B

Target Species:

Human

Cell Background:

Chem-1