Ready-to-Assay CRF2 Corticotropin Releasing Factor Receptor Frozen Cells

Background Information:

The Ready-to-Assay CRF2 Corticotropin Releasing Factor Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. CRH, sauvagine) based activation of CRHR2 GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress CRHR2 alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of CRHR2 , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Corticotropin-Releasing Factor

Sub Family:

Class B

Protein Name:

CRF2

Protein Aliases:

CRF-R2|CRFR-2|Corticotropin-releasing hormone receptor 2|CRH-R-2|CRH-R2

Accession Number:

NM_001883

UniProt Number:

Q13324

Gene Name:

CRHR2

Gene ID:

1395

Gene Aliases:

CRF2|CRF-RB|HM-CRF|CRF2R|CRH2R

Target Species:

Human

Cell Background:

Chem-1