Biological Information

Background Information:

The cAMP Hunter™ GPR84 Gi cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. embelin) based activation of GPR84 GPCR activity via detection of decreasing levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GPR84 , a naturally Gi/Go coupled GPCR. Upon activation of GPR84 , the decrease in levels of intracellular cAMP leads to a decreased signal from EFC when the cAMP detection kit is applied.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Class A Orphan

Sub Family:

Orphan and other 7TM

Protein Name:

GPR84

Accession Number:

NM_020370

UniProt Number:

Q9NQS5

Gene Name:

GPR84

Gene ID:

53831

Gene Aliases:

EX33

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

cAMP Detection

Detection Method:

Chemiluminescence

Additional Information

Brand:

cAMP Hunter™