Biological Information
Background Information:
The cAMP Hunter™ Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Liraglutide) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with Victoza® (not supplied in the bioassay kit). Victoza® is a registered trademark of Novo Nordisk A/S. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.
Target Class:
GPCR
Family:
Glucagon
Sub Family:
Class B
Protein Name:
GLP-1
Protein Aliases:
GLP-1-R|GLP-1R
Accession Number:
NM_002062.3
UniProt Number:
P43220
Gene Name:
GLP1R
Gene ID:
2740
Target Species:
Human
Cell Background:
CHO-K1
Usage
Product Type:
Bioassay Kits
Application:
Potency, Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based, Qualified
Assay Measures:
cAMP Detection
Detection Method:
Chemiluminescence
Bioassay Data
INN Drug Name:
Liraglutide
Qualified With:
Victoza®
Trademark Statement:
Victoza® is a registered trademark of Novo Nordisk A/S
Clinical Relevance
Therapeutic Area:
Metabolic Diseases
Additional Information
Brand:
cAMP Hunter™