Biological Information

Background Information:

The cAMP Hunter™ Liraglutide Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses ligand (e.g. Liraglutide) based activation of GLP-1R GPCR activity via detection of levels of intracellular cAMP. This product uses EFC technology. The cells overexpress wild type GLP-1R, a naturally Gs coupled GPCR. Upon activation of GLP-1R, the increase in levels of intracellular cAMP leads to an increased signal from EFC when the cAMP detection kit is applied. This assay has been optimized and qualified with Victoza® (not supplied in the bioassay kit). Victoza® is a registered trademark of Novo Nordisk A/S. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

CHO-K1

Usage

Product Type:

Bioassay Kits

Application:

Potency, Commercial Release & Stability

Kit Components:

KitComponent

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based, Qualified

Assay Measures:

cAMP Detection

Detection Method:

Chemiluminescence

Bioassay Data

INN Drug Name:

Liraglutide

Qualified With:

Victoza®

Trademark Statement:

Victoza® is a registered trademark of Novo Nordisk A/S

Clinical Relevance

Therapeutic Area:

Metabolic Diseases

Additional Information

Brand:

cAMP Hunter™