cAMP Hunter™ Semaglutide Bioassay Kit

Background Information:

The cAMP Hunter™ Semaglutide bioassay provides a robust, and highly sensitive functional, cell-based assay to monitor 3’-5’-cyclic adenosine monophosphate (cAMP) production in cells, as a result of ligand (e.g. Exendin-4)-mediated activation of the GLP-1 receptor. The bioassay kit contains all the materials needed for a complete assay, including cells, detection reagents, cell plating reagent, positive control agonist, and assay plates. The ready-to-use cryopreserved cells have been manufactured to ensure assay reproducibility and faster implementation from characterization to QC lot-release. This bioassay has been optimized for a 96-well format. Note that control agonist for stimulating the cells, recombinant human Exendin-4, is not included in the kit, and may be purchased separately. This assay has been optimized and qualified with Semaglutide (not supplied in the bioassay kit). Ozempic® is a registered trademark of Novo Nordisk A/S. The cAMP Hunter™ Semaglutide Bioassay Kit is an easy-to-use cell-based assay to measure drug potency and detect neutralizing antibodies.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

GPCR

Family:

Glucagon

Sub Family:

Class B

Protein Name:

GLP-1

Protein Aliases:

GLP-1-R|GLP-1R

Accession Number:

NM_002062.3

UniProt Number:

P43220

Gene Name:

GLP1R

Gene ID:

2740

Target Species:

Human

Cell Background:

CHO-K1