ChemiSCREEN™ 5-HT2A Serotonin Receptor Stable Cell Line

Background Information:

The ChemiSCREEN™ 5-HT2A Serotonin Receptor Stable Cell Line is an engineered cell line for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This cell line enables assessment of ligand (e.g. serotonin/5-HT) based activation of HTR2A GPCR activity via detection of increasing levels of intracellular calcium. The included cell line overexpresses HTR2A alongside endogenous high levels of the promiscuous G protein, Gα15, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2A , the resulting calcium activity can be fluorescently assayed.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Protein Name:

5-HT2A

Protein Aliases:

5-HT-2A|Serotonin receptor 2A

Accession Number:

NM_000621

UniProt Number:

P28223

Gene Name:

HTR2A

Gene ID:

3356

Gene Aliases:

HTR2

Target Species:

Human

Cell Background:

Chem-1