PathHunter® eXpress CDC25A Degradation Assay

Background Information:

The PathHunter® eXpress CDC25A Degradation Assay provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting the cell cycle checkpoint pathway. This kit enables detection of levels of CDC25a, allowing for assessment of CHK-1-activation-induced CDC25a degradation. This product uses EFC technology. The cells included overexpress CDC25a target protein fused to PL. EA is contained in the detection reagent. Upon pathway-activation-induced degradation of CDC25a EFC signal is lost. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Phosphatase

Family:

Dual Specificity

Sub Family:

Protein Tyrosine

Protein Name:

CDC25A

Protein Aliases:

Cell division cycle 25A|Dual specificity phosphatase Cdc25A|MPIP1

Accession Number:

NM_001789.2

UniProt Number:

P30304

Gene Name:

CDC25A

Gene ID:

993

Target Species:

Human

Cell Background:

U2OS