PathHunter® eXpress IL4R/IL2RG Dimerization Assay

Background Information:

The PathHunter® IL4R/IL2RG Dimerization eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting receptor-receptor interaction. This kit assesses ligand (e.g. IL-4) induced dimerization of IL4R/IL2RG receptors. This product uses EFC technology. The cells included overexpress IL4R target protein fused to PK alongside IL2RG target protein fused to EA. Upon ligand-induced dimerization of IL4R/IL2RG, detectable EFC signal is produced. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates.

Target Class:

Cytokine Receptor

Family:

IL-2 Receptor

Protein Name:

IL4RA

Protein Aliases:

IL-4R subunit alpha|IL-4R-alpha|IL-4RA|CD antigen CD124 [Cleaved into: Soluble interleukin-4 receptor subunit alpha|Soluble IL-4 receptor subunit alpha|Soluble IL-4R-alpha|sIL4Ralpha/prot|IL-4-binding protein|IL4-BP]

Accession Number:

NM_000418.3

UniProt Number:

P24394

Gene Name:

IL4R

Gene ID:

3566

Gene Aliases:

IL4RA|582J2.1

Target Species:

Human

Cell Background:

U2OS