PathHunter® Growth Hormone Bioassay Kit

Background Information:

The PathHunter® Growth Hormone Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses human growth hormone-induced activation of GHR cytokine receptors via detection of SH2 domain protein recruitment. The included cells overexpress ProLink-tagged GHR target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal. This assay has been optimized and qualified with hGH (not supplied in the bioassay kit). Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.

Please reach out to Tech Support at DiscoverXSupportUS@discovery.eurofinsus.com for lot specific CoA.

Target Class:

Cytokine Receptor

Family:

Prolactin Receptor

Protein Name:

GHR

Protein Aliases:

Somatotropin receptor [Cleaved into: Growth hormone-binding protein|GH-binding protein|GHBP|Serum-binding protein]

Accession Number:

NM-000163

UniProt Number:

P10912

Gene Name:

GHR

Gene ID:

2690

Gene Aliases:

GHBP

Target Species:

Human

Cell Background:

U2OS