PathHunter® Jurkat ICOS Signaling Assay

Background Information:

The PathHunter® ICOS Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. ICOS-L) based activation of ICOS receptors via detection of SH2 domain protein recruitment. This product uses EFC technology. The included cell line overexpresses PK-tagged ICOS target protein and EA-tagged SH2 domain protein. Upon activation of the receptor by its ligand or an agonist antibody, recruitment of SH2 domain protein to the phosphorylated receptor tail leads to production of EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Checkpoint Receptor

Family:

IgSF

Sub Family:

Immune Co-Stimulatory Receptor

Protein Name:

ICOS

Protein Aliases:

CD antigen CD278

Accession Number:

NM_012092.3

UniProt Number:

Q9Y6W8

Gene Name:

ICOS

Gene ID:

29851

Gene Aliases:

AILIM|CD278

Target Species:

Human

Cell Background:

Jurkat