PathHunter® NF-kB Pathway Reporter Cell Line (HEK293)
The PathHunter® Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter® Enzyme Fragment Complementation (EFC) technology.
Catalog #: 93-1164C1
Size: 2 vials
Unit Price: Contact For Pricing
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Specifications
Documentation
Required Products
Biological Information
Background Information:
PathHunter® Pathway Reporter cell lines are engineered to express an Enzyme Donor (ED) tagged reporter protein controlled by a pathway-inducible transcriptional response element. Pathway activation results in induced expressions of the ED-tagged protein. Addition of exogenous Enzyme Acceptor (EA), and buffer, lyses the cell and forces complementation of the ED and EA enzyme fragments. This results in the formation of a functional enzyme that hydrolyzes substrate to generate a chemiluminescent signal.
Target Class:
Signaling Pathway
Family:
Tumour Necrosis Factor (TNF)
Accession Number:
NM_003998.4
Target Species:
Human
Cell Background:
HEK293
Usage
Product Type:
Stable Cell Lines
Application:
Signaling Reporters
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Bioassay Data
Trademark Statement:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
PathHunter®