Biological Information

Background Information:

The PathHunter® Signaling Pathway Reporter Assays are stable clonal cell lines that monitor the activation of specific signaling pathways, utilizing the established PathHunter Enzyme Fragment Complementation (EFC) technology. Each stable cell line is engineered to express a synthetic DNA reporter construct, comprising a pathway-specific transcription factor response element that drives expression of ePL-tagged reporter protein. The activated signaling pathway induces expression of the ePL-tagged reporter protein, which is measured by addition of the detection reagent containing EA, resulting in complementation of the two enzyme fragments, and production of an active enzyme that hydrolyzes the substrate to generate a chemiluminescent signal.

Target Class:

Cytokine Receptor

Accession Number:

NM_022148

Target Species:

Human

Cell Background:

REH

Usage

Product Type:

Stable Cell Lines

Application:

Signaling Reporters

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

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Assay Information

Assay Type:

Cell Based

Clinical Relevance

Therapeutic Area:

Inflammation/Allergy

Additional Information

Brand:

PathHunter®