Specifications
Documentation
Biological Information
Background Information:
The PathHunter® IL6RA/IL6ST Bioassay kit provides an easy-to-use cell based assay to measure drug potency and detect neutralizing antibodies. This bioassay assesses IL-6-induced dimerization of IL6RA/IL6ST receptors. This product uses EFC technology. The cell line included overexpresses IL6ST target protein fused to PK alongside IL6R target protein fused to EA. Upon ligand-induced dimerization of IL6RA/IL6ST, detectable EFC signal is produced. Bioassay kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, control agonist, detection reagent, and assay plates.
Target Class:
Cytokine Receptor
Family:
IL-6 Receptor
Protein Name:
IL6RA
Protein Aliases:
IL-6R subunit alpha|IL-6R-alpha|IL-6RA|IL-6R 1|Membrane glycoprotein 80|gp80|CD antigen CD126
Accession Number:
NM_000565.3
UniProt Number:
P08887
Gene Name:
IL6R
Gene ID:
3570
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Bioassay Kits
Application:
Potency, Commercial Release & Stability
Kit Components:
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Dimerization
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Inflammation/Allergy
Additional Information
Brand:
PathHunter®