PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay

Background Information:

The PathHunter® U2OS KCNH2(G601S) MEM-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting KCNH2. This cell line enables assessment of chaperone-based stabilization of KCNH2(G601S) via detection of rescued membrane trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant KCNH2(G601S), which is misfolded and retained in the ER. The cell line also expresses membrane-localized EA. When a pharmacochaperone stabilizes the mutant KCNH2, it is trafficked to the membrane, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Ion Channel

Family:

Potassium

Sub Family:

Voltage-Gated

Protein Name:

hERG

Protein Aliases:

Ether-a-go-go-related gene potassium channel 1|ERG-1|Eag-related protein 1|Ether-a-go-go-related protein 1|H-ERG|hERG-1|hERG1|Voltage-gated potassium channel subunit Kv11.1

Accession Number:

NM_000238

UniProt Number:

Q12809

Gene Name:

KCNH2

Gene ID:

3757

Gene Aliases:

Kv11.1|HERG|erg1

Target Species:

Human

Cell Background:

U2OS