Biological Information

Background Information:

The PathHunter® U2OS MC4R(T162I) ENDO-EA Pharmacotrafficking Assay is a stable, engineered cell line for use in studying pharmacochaperones targeting MC4R. This cell line enables assessment of chaperone-based stabilization of MC4R(T162I) via detection of rescued membrane-endosome trafficking. This product uses EFC technology. The included cell line overexpresses PK-tagged mutant MC4R(T162I), which is misfolded and retained in the ER. The cell line also expresses endosome-localized EA. When a pharmacochaperone stabilizes the mutant MC4R, it is trafficked to the membrane and then internalized to the endosome, producing EFC signal.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

GPCR

Family:

Melanocortin

Sub Family:

Class A

Coupling:

Gs

Protein Name:

MC4

Accession Number:

NM_005912.2

UniProt Number:

P32245

Gene Name:

MC4R

Gene ID:

4160

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Pharmacotrafficking

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Pharmacotrafficking

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Inflammation/Allergy

Additional Information

Brand:

PathHunter®