Biological Information

Background Information:

The PathHunter® NIK Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of ligand (e.g. CD40, TWEAK) induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein. Upon activation of NFκB signaling by a ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA.
This stable engineered cell line is provided as two vials of cryopreserved cells.

Target Class:

Kinase

Family:

STE

Sub Family:

Protein Ser/Thr

Protein Name:

NIK

Protein Aliases:

serine/threonine protein-kinase

Accession Number:

NM_003954.3

UniProt Number:

Q99558

Gene Name:

MAP3K14

Gene ID:

9020

Gene Aliases:

NIK|HSNIK|FTDCR1B|HS

Target Species:

Human

Cell Background:

U2OS

Usage

Product Type:

Stable Cell Lines

Application:

Drug Discovery & Development

Storage Conditions:

Store in vapor phase of liquid nitrogen.

Usage disclaimer:

These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.

Assay Information

Assay Type:

Cell Based

Assay Measures:

Signaling

Detection Method:

Chemiluminescence

Clinical Relevance

Therapeutic Area:

Oncology/Immuno-Oncology

Additional Information

Brand:

PathHunter®