Biological Information
Background Information:
The PathHunter® OX40 Checkpoint Signaling cell line is a stable, engineered cell line for use in studying drug candidates (small molecules or biologics) targeting signaling checkpoints. This cell line enables assessment of OX40 induced non-canonical NF-κB signaling via detection of NIK stabilization. This product uses EFC technology. The included cell line overexpresses ED-tagged NIK (NF-kappa-B Inducing Kinase) protein as well as untagged OX40 receptor. Upon activation of the receptor by its ligand or an antibody, NIK is stabilized, allowing detection of EFC signal when treated with exogenous EA.
This stable engineered cell line is provided as two vials of cryopreserved cells.
Target Class:
Checkpoint Receptor
Family:
Tumour Necrosis Factor (TNF)
Protein Name:
OX40
Protein Aliases:
OX40L receptor|TAX transcriptionally-activated glycoprotein 1 receptor|CD antigen CD134
Accession Number:
NM_003327.3
UniProt Number:
P43489
Gene Name:
TNFRSF4
Gene ID:
7293
Gene Aliases:
TXGP1L
Target Species:
Human
Cell Background:
U2OS
Usage
Product Type:
Stable Cell Lines
Application:
Drug Discovery & Development
Storage Conditions:
Store in vapor phase of liquid nitrogen.
Usage disclaimer:
These products may be covered by issued US and/or foreign patents, patent applications and subject to a Limited Use Label License. Please visit discoverx.com/license for a list of products that are governed by limited use label license terms and relevant patent and trademark information.
Assay Information
Assay Type:
Cell Based
Assay Measures:
Signaling
Detection Method:
Chemiluminescence
Clinical Relevance
Therapeutic Area:
Oncology/Immuno-Oncology
Additional Information
Brand:
PathHunter®