Ready-to-Assay 5-HT2A Serotonin Receptor Frozen Cells

Background Information:

The Ready-to-Assay 5-HT2A Serotonin Receptor Frozen Cells are a simple, thaw-and-assay cell format for use in studying drug candidates (small molecules or biologics) targeting GPCRs. This assay assesses ligand (e.g. serotonin/5-HT) based activation of HTR2A GPCR activity via detection of increasing levels of intracellular calcium. The included cells overexpress HTR2A alongside endogenous high levels of a promiscuous G-protein, coupling the receptor to the calcium signaling pathway. Upon activation of HTR2A , the resulting calcium activity can be fluorescently assayed. Ready-to-assay kits consist of two vials of the stably modified clonal cell line in a convenient thaw-and-use format.

Target Class:

GPCR

Family:

Serotonin

Sub Family:

Class A

Protein Name:

5-HT2A

Protein Aliases:

5-HT-2A|Serotonin receptor 2A

Accession Number:

NM_000621

UniProt Number:

P28223

Gene Name:

HTR2A

Gene ID:

3356

Gene Aliases:

HTR2

Target Species:

Human

Cell Background:

Chem-1