Recombinant Protein Capabilities

Accelerate Drug Discovery with Reliable and High-quality Proteins – Better Targets for Better Drugs

Producing high-quality, functional proteins for hit finding, antibody development, and structural studies is a complex process requiring precision, scientific expertise coupled with innovative techniques, and seamless project coordination. Trust Eurofins CALIXAR’s protein development capabilities to deliver high-quality functional recombinant proteins for your early drug discovery programs and save time by significantly increasing your drug targets’ chances of clinical trials success.

Eurofins CALIXAR, the protein center of excellence part of Eurofins DiscoverX®, offers fully integrated custom protein production capabilities that can handle every stage of protein development in-house — from construct design to expression, purification, and functional validation. This approach assures that high-quality, functional proteins are delivered, eliminating the need for rework or switching providers. Streamlined processes ensures that the native fold, any post-translational modifications, and protein functionality are preserved within the native binding pockets and epitopes are maintained.

Eurofins CALIXAR recombinant protein capabilities team specializes in producing a wide range of proteins, including membrane proteins (such as GPCRs, ion channels, and transporters) and soluble proteins (like kinases, phosphatases, epigenetic proteins, and other enzymes), supporting various applications for both small molecule (hit identification and screening), large molecules (antibody development including immunization and library screening), and 3D structure-based drug design. With over a decade of experience, Eurofins CALIXAR’s advanced technologies and scientific expertise ensure consistent quality and reliable recombinant protein expression, minimizing risks and enabling your research and drug discovery to stay on track.

Product Highlights
  • Preserve Native Folding and Functionality – Proprietary processes produce full-length, unmutated proteins with intact conformation
  • Expertise in Challenging Protein Targets – 750+ successful projects including complex proteins (e.g., GPCRs, ion channels) and large protein complexes of multiple subunits
  • Innovative Stabilization and Solubilization – Leveraging methods like nanodiscs, amphipols, polymers, and proprietary detergents to ensure membrane proteins remain stable and functional under biologically relevant conditions
  • Versatility for Key Applications – Supporting small molecule screening, structural biology studies, bioassay development, and antibody discovery tailored to drive success in diverse research programs
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Expertise & Capabilities

Eurofins CALIXAR’s custom protein expression capabilities provide end-to-end support — from construct design to final delivery — ensuring every protein is tailored to meet the unique demands of your project. Leveraging advanced expression systems and specialized methodologies produces proteins with preserved structure, biological activity, and functional integrity.
 

The most challenging targets in drug discovery, bioassay development, and structural studies can be addressed for both membrane and soluble proteins. Workflow steps are meticulously optimized to ensure high-quality outcomes for even the most complex protein targets, and this streamlined project approach ensures that each protein is fit-for-purpose, whether for high-throughput screening, structural analysis, or antibody discovery.
 

Key Capabilities

  • Advanced Membrane Protein Solutions: Production of functional GPCRs, ion channels, and transporters with full-length, stable, and native conformations
  • Development of Soluble Proteins: Expertise in enzymes, kinases, and phosphatases for applications like enzymatic assays, mechanistic studies, and drug screening
  • Custom Protein Design: Designing proteins with native folding, intact binding sites, and functional activity to support early drug discovery, hit identification, and lead optimization
  • Custom Formats and Delivery: Tailored production of proteins in formats such as nanodiscs, proteoliposomes, or other specific formats adapted to your research needs

Project Approach

With over 750 successfully completed projects and more than a decade of experience, Eurofins CALIXAR’s custom protein expression capabilities are tailored to address the most complex research challenges. Our approach is founded on five essential pillars, ensuring precision, reproducibility, and scientific excellence at every stage.
 

  1. Native Protein Stability
    • Preserved Native Structure: Proprietary stabilization technologies ensure full-length proteins maintain their native conformations and biological activities
    • o High Purity & Reproducibility: Production of proteins with >90% purity, validated for stability and reproducibility across multiple applications
  2. Scientific Expertise
    • Specialized Scientific Leadership: Protein development is led by scientists with extensive experience in working with complex targets
    • Proven Track Record: Demonstrated success with GPCRs, ion channels, kinases, and viral proteins, enabling hit identification, lead optimization, and early drug discovery
  3. Streamlined Project Management
    • Efficient Workflow: Milestone-driven processes with kickoff meetings, defined project phases, and regular progress updates
    • Dedicated Project Support: Each project is supervised by a dedicated project manager to ensure timely delivery and clear communication
  4. Global Reach & Innovation
    • Global Network of Resources: As part of the Eurofins group, we leverage global scientific resources and advanced R&D infrastructure
  5. Rigorous Quality Standards:
    • Comprehensive Quality Control: QC processes include structural validation, functional assays, and consistency checks for batch-to-batch reproducibility
    • Application-ready Proteins: Proteins are produced to meet the highest standards for structural biology, small molecule screening, and antibody discovery

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Eurofins CALIXAR’s extensive expertise in protein development, highlighting the 500+ soluble proteins and 250+ membrane proteins successfully produced. For soluble proteins, expertise covers a wide range of targets, including kinases like MAPK2, CK2, and NEK2; epigenetic proteins such as CD4 and MKP5; and phosphatases like JMJD and DNMT1. For membrane proteins, expertise covers ion channels like Nav1.7, transporters such as AcrB and BmrA, and GPCRs like A2AR and MT1R/MT2R. Kinases constitute 84% of the soluble proteins, while GPCRs account for 22% of the membrane proteins generated.
 

With a proven track record in complex protein development, Eurofins CALIXAR provides specialized solutions for diverse research applications and ensures that each project is approached with scientific rigor, upholding the highest standards of quality, purity, and functional integrity. Start your recombinant protein expression journey today — Contact us to discuss your project and turn your vision into reality.