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PATHscan - Target-Specific Signaling Pathway Panel

LeadHunter® Discovery Services provides you access to a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to compound treatment. Based on the proven PathHunter® technology, these stable cell lines allow you to measure distinct events within a variety of pathways involved in compound toxicity, cholesterol metabolism, antioxidant pathways, DNA damage, ER stress and many others to drive your lead discovery and optimization without relying on reporter gene assays or complex phenotypic screens. 

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PathHunter® Pathway Assays are novel, non-imaging functional whole cell assays for measuring multiple cellular signaling events such as protein translocation, secretion, degradation and expression using highly sensitive and quantitative chemiluminescent detection. This approach can be applied to any cell signaling cascade in which a target protein either degrades, translocates or is secreted. These assays serve as pathway indicators.

Learn more about PathHunter® Pathway Assays >

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Panel Highlights and Benefits

  • Functionally validated cell lines – high quality results every time
  • Single functional readout – applicable to different kinase receptors and families
  • High quality, reproducible data
  • Superior assay windows & robust performance (Z' > 0.6)
  • Agonist and antagonist modes available
  • Broad applications – specificity and compound toxicity/liability
  • Rapid data turnaround time

Screen for Activators or Inhibitors of PI3-K/AKT Signalling



PathHunter® assays are able to measure FOXO translocation in response to PI3 or AKT inhibition.

Flexible Screening & Profiling Options

LeadHunter Discovery Services reduces the time and costs associated with drug discovery. Whether you are interested in full panel screening and profiling, or selecting a subset of assays, we offer the selection and flexibility to support your drug discovery needs.
 

Overview of PATHscanPanels & Services

Services Assays Pre-configured Panel Customizable? Standard Turnaround
pathELECT 19 No Yes 3 weeks
pathE/IC50ELECT 19 No Yes 3 weeks
pathMAX 13 Yes No 4 weeks
Although standard turnaround times for ELECT assays are 3 weeks, express turnaround times of 10 days can be requested.

pathELECT

A flexible approach to personalized screening
Select only the pathway assay you need. An ideal solution for any size compound collection.
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pathE/IC50ELECT

Quantitative binding affinity measurements
Quantitative affinity measurement (10-point dose-response curve, performed in duplicate) can be determined for any pathway assay/compound combination.
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pathMAX

Quickly profile compounds across multiple signaling pathways
13 functional cell-based pathway assays to gain insight into mechanism of action
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PATHscan Assay List

Below is a complete list of available PATHscan assays.  The list can be sorted by "Target Gene", "Gene ID", "GeneBank Accession Number", or "Species".


Target Gene ▲Common NameFamilyAccession No.
BCL2-BAXBCL2 B-cell CLL/lymphoma 2 (Bcl-2)BCL2NM_000633
BCL2-BIMBCL2 B-cell CLL/lymphoma 2 (Bcl-2)BCL2NM_000633
BCL2L1-BAXBCL2-like 1 (Bcl-xL)BCL2NM_138578.1
BCL2L1-BIMBCL2-like 1 (Bcl-xL)BCL2NM_138578.1
CDC25ACell division cycle 25 homolog A (CDC25A)Cell Cycle ProteinNM_001789.2
FOXO3forkhead box O3 (forkhead box O3)PathwayNM_001455
IκBnuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)PathwayNM_020529
Keap1-NRF2nuclear factor (erythroid-derived 2)-like 2 (NFE2L2)Transcription FactorNM_006164
MCL1-BIMMyeloid cell leukemia sequence 1 (Mcl-1)BCL2NM_021960
NIKmitogen-activated protein kinase kinase kinase 14 (NIK)STENP_003945.2
NPR1Natriuretic peptide receptor A (NPR1)Guanylate Cyclase ANM_000906.3
P53P53 (P53)Nuclear TranslocationNM_000546.2
RANK-IκBTumor necrosis factor receptor superfamily, member 11a (TNFRSF11A)Tumor Necrosis Factor Receptor SuperfamilyNM_003839.3
RELA-IκBv-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA)Transcription factorNM_021975
SREBP2Sterol regulatory element binding transcription factor 2 (SREBF2)Transcription FactorNM_004599.2
TORC1CREB regulated transcription coactivator 1 (CRTC1)Transcription factorNM_015321.1
TORC2CREB regulated transcription coactivator 2 (CRTC2)Transcription factorNM_181715
XBP1X-box binding protein 1 (XBP1)Transcription factorBC012841
β-CateninCatenin (cadherin-associated protein), beta 1 (CTNNB1)Wnt-Frizzled signallingNM_001904