PATHscan^℠ - Target-Specific Signaling Pathway Panel

LeadHunter^® Discovery Services provides you access to a comprehensive collection of cell-based pathway indicator assays designed to detect activation or inhibition of complex signal transduction pathways in response to compound treatment. Based on the proven PathHunter^® technology, these stable cell lines allow you to measure distinct events within a variety of pathways involved in compound toxicity, cholesterol metabolism, antioxidant pathways, DNA damage, ER stress and many others to drive your lead discovery and optimization without relying on reporter gene assays or complex phenotypic screens.

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PathHunter^® Pathway Assays are novel, non-imaging functional whole cell assays for measuring multiple cellular signaling events such as protein translocation, secretion, degradation and expression using highly sensitive and quantitative chemiluminescent detection. This approach can be applied to any cell signaling cascade in which a target protein either degrades, translocates or is secreted. These assays serve as pathway indicators.

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Panel Highlights and Benefits

Functionally validated cell lines – high quality results every time
Single functional readout – applicable to different kinase receptors and families
High quality, reproducible data
Superior assay windows & robust performance (Z' > 0.6)
Agonist and antagonist modes available
Broad applications – specificity and compound toxicity/liability
Rapid data turnaround time

Screen for Activators or Inhibitors of PI3-K/AKT Signalling

PathHunter® assays are able to measure FOXO translocation in response to PI3 or AKT inhibition.

Flexible Screening & Profiling Options

LeadHunter Discovery Services reduces the time and costs associated with drug discovery. Whether you are interested in full panel screening and profiling, or selecting a subset of assays, we offer the selection and flexibility to support your drug discovery needs.

Overview of PATHscan℠ Panels & Services

Services	Assays	Pre-configured Panel	Customizable?	Standard Turnaround
pathELECT	19	No	Yes	3 weeks
pathE/IC50ELECT	19	No	Yes	3 weeks
pathMAX	13	Yes	No	4 weeks

Although standard turnaround times for ELECT assays are 3 weeks, express turnaround times of 10 days can be requested.

pathELECT

A flexible approach to personalized screening

Select only the pathway assay you need. An ideal solution for any size compound collection.

pathE/IC50ELECT

Quantitative binding affinity measurements

Quantitative affinity measurement (10-point dose-response curve, performed in duplicate) can be determined for any pathway assay/compound combination.

pathMAX

Quickly profile compounds across multiple signaling pathways

13 functional cell-based pathway assays to gain insight into mechanism of action

PATHscan Assay List

Below is a complete list of available PATHscan assays. The list can be sorted by "Target Gene", "Gene ID", "GeneBank Accession Number", or "Species".

Target Gene ▲	Common Name	Family	Accession No.
BCL2-BAX	BCL2 B-cell CLL/lymphoma 2 (Bcl-2)	BCL2	NM_000633
BCL2-BIM	BCL2 B-cell CLL/lymphoma 2 (Bcl-2)	BCL2	NM_000633
BCL2L1-BAX	BCL2-like 1 (Bcl-xL)	BCL2	NM_138578.1
BCL2L1-BIM	BCL2-like 1 (Bcl-xL)	BCL2	NM_138578.1
CDC25A	Cell division cycle 25 homolog A (CDC25A)	Cell Cycle Protein	NM_001789.2
FOXO3	forkhead box O3 (forkhead box O3)	Pathway	NM_001455
IκB	nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha (NFKBIA)	Pathway	NM_020529
Keap1-NRF2	nuclear factor (erythroid-derived 2)-like 2 (NFE2L2)	Transcription Factor	NM_006164
MCL1-BIM	Myeloid cell leukemia sequence 1 (Mcl-1)	BCL2	NM_021960
NIK	mitogen-activated protein kinase kinase kinase 14 (NIK)	STE	NP_003945.2
NPR1	Natriuretic peptide receptor A (NPR1)	Guanylate Cyclase A	NM_000906.3
P53	P53 (P53)	Nuclear Translocation	NM_000546.2
RANK-IκB	Tumor necrosis factor receptor superfamily, member 11a (TNFRSF11A)	Tumor Necrosis Factor Receptor Superfamily	NM_003839.3
RELA-IκB	v-rel reticuloendotheliosis viral oncogene homolog A (avian) (RELA)	Transcription factor	NM_021975
SREBP2	Sterol regulatory element binding transcription factor 2 (SREBF2)	Transcription Factor	NM_004599.2
TORC1	CREB regulated transcription coactivator 1 (CRTC1)	Transcription factor	NM_015321.1
TORC2	CREB regulated transcription coactivator 2 (CRTC2)	Transcription factor	NM_181715
XBP1	X-box binding protein 1 (XBP1)	Transcription factor	BC012841
β-Catenin	Catenin (cadherin-associated protein), beta 1 (CTNNB1)	Wnt-Frizzled signalling	NM_001904

PATHscan℠ - Target-Specific Signaling Pathway Panel